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A Low Voltage Wiring System for an Office Block
Nestrojil, Martin ; Slavata, Petr (referee) ; Kadlec, Radim (advisor)
This paper describes the design of a low voltage network in an office building. In the beginning work is given for the theoretical part of the topics that we need to create a network design in a given building. In this part of the guiding principles are conductor sizing, fuse wires and a selection of protective devices. Everything was sought in scientific publications and Articles. The theoretical part will be followed by a practical part with the first adjusted underlying schema, and then will address a practical design using programs supplied by the company Eaton. The goal of this work is to get acquainted theoretically with the main principles of network design, and then practically with your programs and create a complete network design of the building.
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Implementation of kinematics algorithms for the four legged mobile robot
Králík, Jan ; Appel, Martin (referee) ; Sova, Václav (advisor)
The goal of the work is restore a quadruped walking spider-type robot named Jaromír. Part of the work is a body design that is then printed on a 3D printer. Another part is the design and creation of a new control board, which’ll include sensor equipment appropriate for robot operation. Subsequently, the thesis deals with the design of the tactile sensor’s and their fitting on the legs. The final part is implementation of the algorithm for robot control. The goal was to put the model to a state where it could be used for learning and presentations.
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Karyotype evolution of selected families of entelegyne spiders
Kotz, Matěj ; Král, Jiří (advisor) ; Nguyen, Petr (referee)
The Araneoidea superfamily is a diverse clade of spiders with a great species diversity. The whole superfamily displays considerable conservativeness of observed karyotypes. Most likely ancestral karyotype in males is 24 acrocentric chromosomes with X1X2 sex determination system. The goal of this study is to explore the karyotype diversity of two araneoid families - Araneidae and Mimetidae. The majority of studied species exhibit the ancestral karyotype. In some species of the aformentioned families was observed sudden increase in chromosome numbers, up to 2n♂ = 52 in Araneidae and up to 2n♂ = 57 in Mimetidae. The latter number is the highest chromosome count observed in Entelegynae so far. Increase in 2n goes hand in hand with increase in sex chromosome numbers, leading up to X1X2X3X40 system in Araneidae and up to X1X2X3X4X5X6X70 in Mimetidae. I suggest polyploidy as a possible mechanism of the increase. To test this hypothesis, I measured the size of the genome using flow cytometry and used fluorescence in situ hybridization for the detection of 18S rRNA and 5S rRNA genes. For one species, probe for U2 snRNA gene was also optimized as part of this thesis. In many species studied, these techniques were used for the first time ever. In the case of the family Mimetidae, the largest genomes in...
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Impact of endosymbiotic bacteria on the capacity of host spiders (Araneae) to provide ecosystem services
Gloríková, Nela ; Řezáč, Milan (advisor) ; Tropek, Robert (referee)
The impact of the microbial community as well as individual strains of endosymbiotic bacteria on spider hosts has only begun to be increasingly studied in the last 20 years. This is primarily due to new molecular methods and devices, but on the other hand also to a growing desire to unravel the mechanisms behind the ability of spiders to provide ecosystem services. Their unique contribution lies in their high diversity, their generalist hunting strategy, and their efficient long-distance dispersal called ballooning. However, their abilities are possibly affected by the tiny organisms inhabiting their tissues - from the haemolymph to the digestive tract and the reproductive organs. In this work, attention is paid to the aspects that are influenced or manipulated by microbes for the purpose of their own transmission. Studies investigating known reproductive manipulators such as Wobachia, Cardinium or Rickettsia are summarized. The work summarizes recent findings in the areas of the impact of endosymbiotic bacteria on metabolic pathways and nutritional support, but also on behavioral aspects such as predatory/antipredatory behavior, defense against parasitoids or pathogens, and dispersal or avoidance of stress. Information about spiders is also related to knowledge about other invertebrates. Keywords:...
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Aphidophagy among polyphagous predators
Valdecká, Miriam ; Řezáč, Milan (advisor) ; Dolejš, Petr (referee)
Aphids are among the most widespread insect pests in agroecosystems. So far, significant attention has been paid to their natural enemies, especially to specialized predators and parasitoids. There is less information about the influence of polyphagous predators, especially spiders and ground beetles. Therefore, the aim of this thesis is to summarize the knowledge about this topic. And focus on the advantages and disadvantages of aphidophagy in polyphagous predators. Key words: Aphid, aphidophagy, polyphagous, spider, carabid, predator
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Karyotype evolution of African clades of theraphosid mygalomorphs
Košátko, Prokop ; Král, Jiří (advisor) ; Nguyen, Petr (referee)
Karyotypes of mygalomorph spiders are not satisfactorily known. This thesis is focused on the basic cytogenetic analysis of selected species of African clades of theraphosid mygalomorphs. It includes four subfamilies: Eumenophorinae, Harpactirinae, Ischnocolinae and Stromatopelminae. Diploid numbers, chromosome morphology, sex chromosome systems and chromosome behaviour in male germline in the selected species of African theraphosid subfamilies were studied. The findings support published results, that refer of high karyotype diversity in Theraphosidae. Diploid chromosome number reduction is probably a basic trend of theraphosid karyotype evolution. The majority of analysed species exhibited one, two or three sex chromosomes. In some species neo-sex chromosome systems were found. In some species one or two sex chromosome pairs (SCP), composed of chromosomes which lack morphological differentiation were detected. Nucleolus organizer regions were detected by fluorescent in situ hybridization in several species. Constitutive heterochromatin detection was performed by C-banding in two species. Keywords: constitutive heterochromatin, diploid number, karyotype, fluorescence in situ hybridization, Mygalomorphae, nucleolus organizer region, SCP, sex chromosome, spider, Theraphosidae
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Karyotype analysis of mygalomorph spiders of the families Hexathelidae and Dipluridae
Hrubá, Barbora ; Král, Jiří (advisor) ; Sember, Alexandr (referee)
Cytogenetics of spiders of the infraorder Mygalomorphae is largely unknown. My thesis is focused on the karyotype evolution of spiders of families Hexathelidae Dipluridae, which are basal groups of the superfamily Avicularioidea. In this thesis the evolution of diploid chromosome number, chromosome morfology, and also sex chromosomes are studied. The karyotypes of the mygalomorphs are quite interesting for their diversity. Many members of the family Hexathelidae exhibit extreme systems of a chromosomal sex determination which are composed by many gonosomes X. The meiotic division of males is analyzed. Some species exhibit modifications of this division such as achiazmatic meiosis. Selected markers have been detected by fluorescence in situ hybridization. Keywords: evolution, fluorescent in situ hybridization, karyotype, nucleolus organizer region, sex chromosomes, spider
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Karyotype analysis of mesothelid spiders
Prokopcová, Lenka ; Král, Jiří (advisor) ; Zrzavá, Magda (referee)
Cytogenetics of mesothelid spiders is largely unkown. The presented diploma thesis is focused on the karyotype evolution of these spiders. As it is the most basal group of spiders, the analysis of its cytogenetics can bring important data about ancestral spider karyotype. In the framework of my thesis, I analysed diploid chromosome numbers, chromosome morphology, meiotic division, sex chromosomes and the pattern of selected molecular markers that were detected by fluorescence in situ hybridization. According to my results, mesothelid spiders have a high number of chromosomes and the prevalence of monoarmed chromosomes. Unlike other spiders, mesothelids have little differentiated sex chromosomes. Key words: evolution, spider, chromosome, karyotype, fluorescence in situ hybridization, nucleolar organiser region, sex chromosomes
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Karyotype evolution of selected families of entelegyne spiders
Kotz, Matěj ; Král, Jiří (advisor) ; Nguyen, Petr (referee)
The Araneoidea superfamily is a diverse clade of spiders with a great species diversity. The whole superfamily displays considerable conservativeness of observed karyotypes. Most likely ancestral karyotype in males is 24 acrocentric chromosomes with X1X2 sex determination system. The goal of this study is to explore the karyotype diversity of two araneoid families - Araneidae and Mimetidae. The majority of studied species exhibit the ancestral karyotype. In some species of the aformentioned families was observed sudden increase in chromosome numbers, up to 2n♂ = 52 in Araneidae and up to 2n♂ = 57 in Mimetidae. The latter number is the highest chromosome count observed in Entelegynae so far. Increase in 2n goes hand in hand with increase in sex chromosome numbers, leading up to X1X2X3X40 system in Araneidae and up to X1X2X3X4X5X6X70 in Mimetidae. I suggest polyploidy as a possible mechanism of the increase. To test this hypothesis, I measured the size of the genome using flow cytometry and used fluorescence in situ hybridization for the detection of 18S rRNA and 5S rRNA genes. For one species, probe for U2 snRNA gene was also optimized as part of this thesis. In many species studied, these techniques were used for the first time ever. In the case of the family Mimetidae, the largest genomes in...
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Aphidophagy among polyphagous predators
Valdecká, Miriam ; Řezáč, Milan (advisor) ; Dolejš, Petr (referee)
Aphids are among the most widespread insect pests in agroecosystems. So far, significant attention has been paid to their natural enemies, especially to specialized predators and parasitoids. There is less information about the influence of polyphagous predators, especially spiders and ground beetles. Therefore, the aim of this thesis is to summarize the knowledge about this topic. And focus on the advantages and disadvantages of aphidophagy in polyphagous predators. Key words: Aphid, aphidophagy, polyphagous, spider, carabid, predator
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